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Organotypic Hippocampal Slice Cultures - a 2 minute Preview of the Experimental Protocol

Ximena Opitz-Araya, Andres Barria
University of Washington School of Medicine, Department of Physiology and Biophysics;

We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.

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