Nitrogen Estimation Of Organic Compound Through KJELDAHL Method|Detection Of N2 In Organic Compound

Nitrogen Estimation Of Organic Compound Through KJELDAHL Method|Detection Of N2 In Organic Compound


   / @vikrantsingh5008  


Kjeldal Method is a lab analysis method that is use to analysis of percentage of nitrogen in nitrogenous Organic compound like protein Urea amino acid etc first devloped of kjeldal method was Denis chemist johan Kjeldal In this method first digestion of Organic compound and then distillation and then Titration

Requirements

Digestion flask
Kjeldal appratus
organic compound sample
distilled water
sulphuric acid
cupper sulphate
pottasium sulphate
sodium hydroxide
boric acid
dil. HCL and mix indicator


procedure

First take 1 gm organic sample in digestion flask and add 1 gm cupper sulphate and 8 gm pottasium sulphate and 20 ml sulphuric acid and digest at 80-90 digree centigrade for 50 to 60 min after digestion cooled down sample and add 60 ml distilled water and 40 percent 50 Sodium hydroxide solution and keep the distillation for 50 min in distillation step release amonia gas they cooled and absorbed in dispenser they contain boric acid solution after distillation take boric acid solution And Titration with dilute HCL and add mix indicator the end poin Titration colur of boric acid solution change to greenish to pinkish then Titration stop and notedown burate reading how much acid consume and burate reading put the formula and nitrogen quantity calculate please watch Full video and please like and subscribe Thankyou.


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