Скачать или смотреть Intestinal Organoid Generation & hiPSC Maintenance - Comparative Study of Animal ECM and VitroGel®

Webinar Abstract:
Comparative Study of Animal-derived ECMs and VitroGel® Hydrogel for hiPSC Maintenance and Intestinal Organoid Generation

Organoids, self-organizing tiny versions of organs, can be grown from tissue samples and induced pluripotent stem cells (iPSCs). The generation of organoids requires physiological cues from the extracellular matrix (ECM) not present in classical cell culture materials. Commercially available matrix systems derived from animal sources (e.g., Matrigel®, Cultrex™, Geltrex™) and xeno-free hydrogel (e.g., VitroGel®) are widely used in iPSC and organoid research. The benefits and setbacks associated with their intrinsic properties have been individually noted by many. We performed a comparative study of human iPSC maintenance and intestinal organoids (hIO) generation in three different commercial animal-based matrices: Matrigel, Geltrex, and Cultrex, and an animal-free synthetic hydrogel system, VitroGel.

Join Dr. Janny Pineiro-Llanes from the Department of Pharmaceutics at the University of Florida to present their findings.

Key Takeaways:
• Full xeno-free organoid workflow from IPS maintenance to organoid formation.
• Understand the effects of ECM supplements on stem cell growth.