Jamie Hayden - 2-Photon Microscopy: How Deep Can You Go? part 2

Widefield, fluorescence microscopy lets you see everything in
your biological sample, but a lot of it is out of focus, especially
at higher magnifications. Confocal microscopy makes it all
sharp, but limits you to about 150 microns in depth. 2-Photon
microscopy allows you to image routinely to 300-500 microns,
providing a much better 3-dimensional re-creation. In addition,
2-photon does less damage to living cells, so it can be a better
choice for intra-vital and live-cell imaging. This talk will
provide an overview of the experiments, techniques and results
we have obtained in our lab, which generally concentrates on
cancer-related specimens - both live and fixed.

This is the second part of a presentation given at BIOCOMM 2018 in Savannah, Georgia.