Oligonucleotide Ligation Assay(OLA)

Описание к видео Oligonucleotide Ligation Assay(OLA)

It is a method to diagnose hereditary diseases
( Caused by mutation not affecting restriction endonuclease sites.)

This method combines Polymer­ase Chain Reaction with Ligation Assay.

PCR-OLA distinguishes between ligation and absence of ligation of two
oligonucleotides.

A single nucleotide mismatch (at site of hybridized oligonucleotides) prevents
ligation and hence we can distinguish between wild and mutant genotype.

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