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Learning objectives

Describe how an Avidity ELISA work in the context of antibody binding.
Explain what a chotropic agent is.
Explain the purpose of each of these steps:
Adhesive plastic ELISA plate.
Coating the well with antigen protein.
Blocking with bovine albumin.
Plasma incubation which contains the antibody.
Addition of increasing concentrations of a chaotropic agent in different wells.
Detection antibody from another species (e.g. mouse anti-human IgG).
HRP conjugation (joining) to detection antibody.
Colourless substrate peroxidation to coloured product.
Understand a chaotropic agent concentration vs absorbance curve and what it means.