Traction Force Microscopy To Study B Lymphocyte Activation l Protocol Preview

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Traction Force Microscopy to Study B Lymphocyte Activation - a 2 minute Preview of the Experimental Protocol

Anita Kumari, Judith Pineau, Ana-Maria Lennon-Duménil, Martial Balland, Paolo Pierobon
INSERM U932, Institut Curie, PSL Research University; Université Joseph Fourier (Grenoble 1), Laboratoire Interdisciplinaire de Physique;

Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.

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