Скачать или смотреть 🦠 Lentiviral Vector Stable Cell Line Generation using Calcium Phosphate Transfection

NOTE: shRNA allows for stable knockdown of genes while siRNA allows for transient knockdown.

Lentiviral vectors offer stable integration of your gene of interest into the host genome 🧬for greater reproducibility when repeating experiments 🧫involving transgenes. This is in contrast to the transient expression offered by siRNA transfection.

The advantage of lentiviral vectors is that they can transduce both dividing and non dividing cells. The lentivirus vector plasmid is constructed as follows: The gene to be transferred is flanked by viral sequences that can be recognised by viral proteins in a packaging cell line to package the viral genome into viral particles.

Cell lines that are express viral proteins such as the HEK293T, Chinese Hamster Ovaries (CHO) and NIH3T3 allow you to create virions capable of transducing a target cell line. A selection process using either antibiotic resistance or a fluorescent protein reporter, is then used to create a stable line.



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This video is about lentiviral vectors, lentiviral transduction protocol, lentiviral crispr, lentiviral particles, lentiviral packaging, lentiviral production protocol, calcium phosphate transfection, calcium phosphate transfection method, calcium phosphate transfection mechanism, lentiviral vector system, lentiviral vector design, lentiviral vector packaging, lentiviral vector technology, hek293t, hek293t cell line, hek293t transfection and hek293t stable cell line protocol.