FISH to Monitor the Cohesion in Prometaphase and Metaphase I | Protocol Preview

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Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes - a 2 minute Preview of the Experimental Protocol

Adrienne T. Perkins, Sharon E. Bickel
Dartmouth College, Department of Biological Sciences;

This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.

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