Скачать или смотреть What Validation Steps Should You Expect From Your Antibody Manufacturer?

Brief introduction to how CST develops antibody validation protocols.

Transcript

The standards in my view should be...the same standards [that] apply to any good experimental design, which means using appropriate biological systems and positive and negative controls. And these can be in a variety of forms. We use a wide range of them here at CST. And in general, any one single approach is probably not sufficient. It's a combination of those things that's critical. That typically includes positive and negative cell lines or tissues looking at subcellular localization using specific inhibitors or other types of activators to modulate the expression or localization, various forms to either increase or decrease the level of the target protein such as a siRNA or knockout technologies or overexpression. It really depends on the biology and it depends on the tools one has available, but it really boils down to what I consider good experimental design. And when somebody is looking at determining which antibody to use or to purchase a particular antibody, is to actually look at the data that is provided to you and make a decision as if you were reviewing a publication and decide if, in fact, that data is sufficient and it's important to make sure that that data is readily available as well.