Скачать или смотреть Global BioFoundries Alliance Webinar - June 16, 2021

From yeast to mammalian cells: designing and building synthetic eukaryotic chromosomes

Design and large-scale synthesis of DNA has been applied to the functional study of viral and microbial genomes. New and expanded technology development is required to unlock the transformative potential of such bottom-up approaches to the study of larger mammalian genomes. Two major challenges include assembling and delivering long DNA sequences. We have developed a workflow for de novo DNA assembly and delivery that enables functional evaluation of mammalian genes on the length scale of 100 kilobase pairs (kb). The DNA assembly step is supported by an integrated robotic workcell. We envision utility in producing designer variants of human genes linked to disease and their delivery and functional analysis in cell culture or animal models.

Speaker

Leslie Mitchell PhD
Co-founder and President
Neochromosome, Inc.




The StemCellFactory: Automated generation and expansion of human induced pluripotent stem cells

The use of human induced pluripotent stem cells (hiPSCs) offers fascinating perspectives for disease modeling, drug screening and personalized therapy. The globally increasing demand for patient-derived hiPSCs has created an urgent need for standardized and automated production processes. To tackle this challenge, we developed the StemCellFactory, a modular system for automated reprogramming of peripheral blood mononuclear cells (PBMCs) and CRISPR/Cas9-based genome editing of the resulting hiPSCs. Crucial components include a liquid handling unit, a density gradient-station for cell separation, an automated clone picker, high speed microscopy and an Amaxa 4D nucleofector with a 96-well shuttle™, which are all interconnected with a robotic arm to automated incubators, material hotels and analytical tools for cell culture quality control. We expect this automated set-up to largely facilitate the generation of disease-specific and gene-corrected hiPSC lines for disease modelling and drug discovery.

Speakers:

Bastian Nießing
Fraunhofer Institute for Production Technology IPT
Group Manager
Automation in Life Sciences
Department of Production Metrology


Yannik Breitkreuz
LIFE & BRAIN GmbH
Cellomics Unit