Скачать или смотреть How to collect Blood Cultures | Blood Culture Collection Procedure

Drawing blood cultures is a medical procedure performed to identify and diagnose bloodstream infections.

1. Gather the necessary supplies: Wear gloves, and ensure you have the following items: an alcohol swab, a tourniquet, sterile blood culture bottles, sterile needles, a syringe or blood collection tubes, adhesive bandages, and gauze pads.

2. Prepare the patient: Explain the procedure to the patient, ensuring they understand what will happen.

3. Perform hand hygiene: Wash your hands thoroughly with soap and water or use hand sanitizer.

4. Put on gloves: Wear disposable gloves to maintain a sterile environment and protect both you and the patient from potential infections.

5. Apply the tourniquet: Wrap the tourniquet around the patient's upper arm, about 3-4 inches above the intended site of venipuncture.

6. Clean the site: Use an alcohol swab to clean the selected venipuncture site.

7. Prepare the blood culture bottles:


8. Prepare the syringe or blood collection tubes: Attach a sterile needle to the syringe or blood collection tube(s).

9. Perform venipuncture: With one hand, anchor the patient's arm by gently pulling the skin taut below the selected site. Using the other hand, insert the needle into the vein at a shallow angle.

10. Collect blood for the culture: Collect the blood directly into the blood culture bottles or transfer it from the syringe into the bottles.

*The blue (aerobic) blood culture bottle should be filled first, then the purple (anaerobic) bottle is filled second.*

11. Remove the tourniquet and withdraw the needle: Apply gentle pressure with a gauze pad or cotton ball to the venipuncture site to control bleeding.

12. Dispose of the sharps and secure the sample:

13. Post-procedure care: Apply an adhesive bandage to the venipuncture site.

14. Perform Hand hygiene

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