Скачать или смотреть Puromycin biochemistry & uses in biochemistry

Puromycin is really cool. You can use it for selection or to protein-label! I don’t have text written but I just learned about how it works and thought it was cool… blog form: https://bit.ly/puromycin

Here’s a good review article: Aviner R. The science of puromycin: From studies of ribosome function to applications in biotechnology. Comput Struct Biotechnol J. 2020;18:1074-1083. Published 2020 Apr 24. https://dx.doi.org/10.1016%2Fj.csbj.2...

and here’s the paper on QTI-seq, which uses puromycin to identify translation initiation sites: Gao, X., Wan, J., Liu, B. et al. Quantitative profiling of initiating ribosomes in vivo. Nat Methods 12, 147–153 (2015). https://doi.org/10.1038/nmeth.3208

More on ribosome profiling (aka ribosome footprinting aka RiboSeq) here: https://bit.ly/ribosomefootprinting

more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com