BGE Train the trainers - Day 2 (5): DNA pooling and quantification

Описание к видео BGE Train the trainers - Day 2 (5): DNA pooling and quantification

This BGE video outlines the technical skills and theoretical knowledge required for effective DNA pooling and quantification to prepare MinION libraries for DNA barcoding. The principles and procedures involved in pooling DNA samples from multiple sources to create a representative pool for downstream sequencing applications are discussed and demonstrated. Learning outcomes encompass comprehending the importance of accurate DNA quantification in library preparation, interpreting DNA concentration measurements from spectrophotometry and/or fluorometry readings, and troubleshooting common issues encountered during DNA pooling and quantification processes, facilitating the preparation of high-quality MinION libraries for DNA barcoding applications.

Speakers: D. Hall (NHM) & Ben Price (NHM)

This video was recorded during BGE's 'Train the trainers' event at the Aristotle University Thessaloniki, Greece. More information: https://biodiversitygenomics.eu/2023/...

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