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Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays - a 2 minute Preview of the Experimental Protocol

Srikanth Kudithipudi, Denis Kusevic, Sara Weirich, Albert Jeltsch
Stuttgart University, Institute of Biochemistry;

Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

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