Biolayer Interferometry (BLI) | The Biophysics behind the BLI Technology, Explained

Biolayer Interferometry (BLI) technology, central to the Octet® platform, offers a transformative approach to analyzing biomolecular interactions.

BLI is a label-free technology that measures the interference pattern of light reflected from a biological layer, enabling real-time monitoring of molecular binding events.

The Octet® platform's use of BLI technology enhances productivity throughout the drug discovery pipeline from early research and development to manufacturing and quality control, delivering precise and efficient analysis of biomolecular interactions.

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00:02 Biolayer Interferometry or BLI for short, allows users to perform label-free biomolecular interaction analysis in real-time.

00:12 BLI biosensors provide a fluidic-free design facilitating scalability in throughput and capability to assess interactions from crude, unpurified samples during early discovery, development and manufacturing for faster decision making.

00:28 BLI measures light interference originating from the tip of the biosensor surface, where light wavelengths are made to reflect from two layers: a biocompatible layer at the end of the biosensor surface, and an internal reference layer.

00:43 White light that reflects from the two layers contains a mixture of wavelengths that show either constructive, partially constructive, or destructive interference.

00:53 The spectral pattern of the reflected light changes as a function of the optical thickness of the molecular layer and results in a spectral shift

01:02 The interference pattern of this shift is monitored and plotted in a sensorgram in real time.

01:08 This real-time analysis provides precise and accurate data on binding specificities, analyte concentrations and rates of association and dissociation.

01:20 Scalable throughput, flexibility and ease-of-use of the BLI platform give researchers the potential to characterize biomolecular interactions, optimize their bioprocesses and QC studies.

01:34 Biolayer Interferometry has applications throughout the drug discovery pipeline from early research and development to manufacturing and QC.

01:43 It simplifies progress in life sciences and bioprocessing, enabling the development of new and improved therapies in a shorter time-period, decreasing drug to market costs, which leads to more affordable medicines for all.

01:56 Octet® systems based on BLI offer unprecedented time and cost savings during biomolecular interactions analysis

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