Accelerating protein characterization with mass photometry

In this webinar, Refeyn’s Tomás de Garay gives an overview of the principles behind mass photometry, its main applications and the types of molecules it can analyze. He also talks about how mass photometry compares to other analytical techniques such as cryo-EM, SEC, and mass spectrometry. Finally, he discusses technical questions and practical considerations with Scott Hanton, the editorial director of Lab Manager.

TIMESTAMPS

00:00 – Moderator introduces Tomás and Scott
01:20 – Tomás’ talk starts
02:00 – Introduction to mass photometry
05:20 – Mass photometry data example – oligomerization
06:46 – What does a mass photometry measurement look like?
07:50 – Discussion with Scott starts
08:08 – Where does mass photometry fit in a typical biochemical workflow?
11:24 – Is 30 kDa the lower mass limit of mass photometry?
13:11 – How sensitive is the instrument to vibrational or acoustic noise?
14:45 – Discussing the mass resolution of mass photometry
19:48 – What training is required to use mass photometry?
21:24 – How are mass photometry systems typically managed by laboratories?
23:05 – What kind of maintenance do the instruments require?
26:52 – Can mass photometry be automated?
28:52 – Can mass photometry measure macromolecular interactions?
35:35 – Can mass photometry detect changes in a sample over time?
38:10 – How quantitative are mass photometry results?
42:21 – What concentration ranges are optimal for mass photometry?
44:52 – Advantages of mass photometry for measuring protein interactions
46:51 – Can you measure microplastics with mass photometry?
51:05 – Can mass photometry be used for antibody research?
57:18 – Can mass photometry be used in GMP environments?
58:43 – Discussion ends

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Refeyn pioneers analytical instruments that put molecular mass measurement capabilities within easy reach for scientists. Refeyn instruments feature mass photometry technology, which uses light to quantify the mass of single particles in solution without labels. Find our latest video resources on YouTube, including webinars, tutorials, and much more!

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CHECK OUT OTHER REFEYN WEBINARS

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FURTHER RESOURCES:

Brochure – Biomolecular characterization with mass photometry - https://info.refeyn.com/mass-photomet...

Application note – Characterizing protein oligomerization with automated mass photometry - https://www.refeyn.com/app-notes-char...

Application note – Quantifying protein binding affinities using mass photometry - https://www.refeyn.com/app-notes-quan...

Webinar – Rapid analytical approach for biologics characterization – mass photometry   • Rapid analytical approach for biologi...  

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