Скачать или смотреть Virology- Isolation and purification of viruses and component by Dheerendra Kumar

#Virology
#gate biotech
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#biotechnology
#Isolation and purification of viruses and component
#dheerendra kumat tuturial
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#D Kumar Life Science Classes
#students motivation
#Viruses are obligate intracellular parasites that require living cells in order to replicate.
#Cell culture, embryonated eggs and small laboratory animals are used for the isolation of viruses.
#Embryonated eggs are very useful for the isolation of influenza and paramyxoviruses. 
#For primary cell cultures, tissue fragments are first dissociated into small pieces with the help of scissors and addition of trypsin.
The cell suspension is then washed couple of times with minimal essential media and seeded into a flat-bottomed glass or plastic container bottle after resuspending it with a suitable liquid medium and fetal calf serum.
#Consequences of virus infection to animals and human