Скачать или смотреть Sennoside | Industrial Production, Estimation and Utilization (Part 01)

Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - https://play.google.com/store/apps/de...

The leaves are dried and powdered. The powdered drug is shaken with benzene for 2 h on an electronic shaker. Filter and distill off the solvent and marc is dried at room temperature and extracted with 70% methanol for 4–6 hours. The extract is filtered under vacuum and it is extracted with 70% of methanol for 2 h, and filtered. The methanolic extract is combined and concentrated to l/8th portion of its original volume. The concentrated solution is acidified with hydrochloric acid to a pH of 3.2. The acidified solution is kept aside for 2 h at a temperature of 5°C. The solution is filtered and to the filtrate add anhydrous calcium chloride dissolved in 25 ml of denatured spirit with constant and vigorous stirring. The pH is again adjusted to 8 by ammonia and it is set aside for 2 h. The solution is filtered; the precipitate obtained is dried over P2O5 in a dessicator.

Identification tests: To the crude extract, organic solvent like benzene, ether or chloroform is added and shaken. The organic layer is separated and to it ammonia solution is added, the ammoniacal layer produces pink to red colour indicating the presence of anthraquinone glycoside.

Thin Layer Chromatography of Sennosides
Sennosides are spotted in Silica gel-G plates and developed using ethyl acetate: methanol: water (100:16.5:13.5) as solvent system. Red coloured spots will appear when the spots are sprayed with 25% nitric acid and turns to yellow when sprayed after drying with alcoholic potassium hydroxide solution.

Get in touch with the solution by just clicking the following links-

Facebook Group-   / solutionpharamcy  
Mobile Application - https://play.google.com/store/apps/de...
New Channel (Pharmacy Dictionary)    / @pharmacydictionary  
Instagram-   / solutionpharmacy  
E-Mail for official and other work - solutionpharmacy@gmail.com
LinkedIn-   / pushpendrakpatel  
#solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPATonlinetest #GPATclass #GPATvideos #Microbiologyclass#Microbiology#