Laser Microdissection To Uncover Regional Transcriptomics: Human Kidney Tissue l Protocol Preview

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Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue - a 2 minute Preview of the Experimental Protocol

Daria Barwinska, Michael J. Ferkowicz, Ying-Hua Cheng, Seth Winfree, Kenneth W. Dunn, Katherine J. Kelly, Timothy A. Sutton, Brad H. Rovin, Samir V. Parikh, Carrie L. Phillips, Pierre C. Dagher, Tarek M. El-Achkar, Michael T. Eadon,
Indiana University School of Medicine, Department of Medicine; Indiana University School of Medicine, Department of Cellular & Integrative Physiology; Ohio State University Wexner Medical Center, Division of Nephrology, Department of Medicine; Indiana University School of Medicine, Division of Pathology;

We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.

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