From cell seeding to analysis - Getting the best out of your cell-based assay

Presented By:
Jessica Wagener, PhD - Application Specialist Cell Handling, Eppendorf AG

Speaker Biography:
Dr. Jessica Wagener is Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.

Co-Presented By:
Erik Bonke, PhD - Application Specialist, Promega GmbH

Speaker Biography:
Mr. Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.

Webinar:
From cell seeding to analysis - Getting the best out of your cell-based assay

Webinar Abstract:
The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to reliability of experimental data. Despite of choosing the appropriate assay type, there are many more factors to be considered to decrease data variability.
In this webinar Eppendorf and Promega are joining forces to optimize your assay results. We will have a look at the complete cell culture workflow and discuss measures you can take to get the best out of your cell-based assay. Furthermore, practical tips and tricks to avoid common pitfalls will be discussed with one of our most experienced Cell Culture Application Specialists.

In this webinar you will learn:
-How to find the best time point for analysis
-What are the pros/cons of the different modes of detection?
-Tips and tricks for optimal cell culture routine and assay setup
-Tips and tricks for cell-based assay setup

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