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There are different Methods Used For Determination of Vitamin C one such is

Iodimetric method

The conversion of I2 to iodide (I-) is a reversible
reaction. The reduction potential of this system is
around 0.54 V. There are many substances which
have a lower reduction potential than iodine and
such substances have a tendency to lose electrons to
I2 and act as reducing agents.
Such titrations where iodine is used to determine the
concentration of a reducing agent are known as
iodimetric titrations. One such reducing agent is
ascorbic acid and in this method the ascorbic acid
solution is titrated with an iodine solution using
starch as an indicator. In this titration I2 is reduced to
I- and ascorbic acid is oxidized to dehydroascorbic
acid.
Ascorbic acid + Iodine (I2) 2 I− +
Dehydroascorbic acid
When the ascorbic acid is completely oxidized to
dehydroascorbic acid, the excess iodine reacts with
the starch indicator and forms a blue-black starch-
iodine complex. This is the endpoint of the redox
titration. The iodimetric method is simpler than the
potassium iodate method (Satpathy et al., 2021).

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