Released N-Glycan Analysis of Biotherapeutics – Sample Prep & Analysis

This video focuses on released glycan workflows for analysis of biotherapeutics. Dr. Aled Jones addresses the importance of N-Glycan analysis and describes how it works. He then goes on to discuss the importance of choosing the correct dye for released N-glycan analysis. There are three separate types that Dr. Jones covers: InstantPC for HILIC, 2-AB for HILIC, and APTS for CE. InstantPC is a proprietary Agilent dye which provides superior response in both LC/FLD and LC/MS analysis workflows. 2-AB (2-aminobenzamide) is a dye which has been used for decades to label glycans for analysis by LC/FLD and LC/MS. Agilent offers tools and workflows to speed up the historically time-consuming 2-AB sample prep. Finally, APTS (8-aminopyrene-1,3,6-trisulfonic acid is a charged dye intended for separation by capillary electrophoresis with detection by laser-induced fluorescence. Dr. Jones concludes the presentation with sialic acid profiling and quantitation. Terminal sialic acids are often present on glycans in biotherapeutic glycoproteins and can have significant effects on the efficacy of drug products. Agilent offers tools for determining the makeup of different sialic acids (profiling) present by comparison to our Sialic Acid Reference Panel, and for measuring the precise amount of sialic acids present (quantitation).

👉 Learn more about Gly-X technology: https://www.agilent.com/en/product/bi...

👉 Learn more about InstantPC Labeled Glycan Standards and Libraries: https://www.agilent.com/en/product/bi...

👉 Learn more about 2-AB Labeled N-Glycan Standards and Libraries: https://www.agilent.com/en/product/bi...

👉 Learn more about APTS Labeled Glycan Standards: https://www.agilent.com/en/product/bi...
👉 Learn more about Sialic Acid Profiling and Quantitation Glycan Sample Preparation Kits: https://www.agilent.com/en/product/bi...

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