Скачать или смотреть Guy Hermans: Using synthetic antibody libraries to overcome poor immunogenicity

We will discuss llamdA, Isogenica’s massively diverse fully synthetic camelid domain antibody library and Alexandria™, a recently launched synthetic human Fab antibody fragment library. In contrast to immune or even naïve animal derived libraries, the encoded antibody repertoire is not biased by self-tolerance and are therefore more likely to yield high affinity antibodies to well conserved targets or epitopes. Variable region diversity is designed to mimic natural antibody repertoires CDR length and amino acid composition, thereby maximizing the chance of functional folding. Despite this similarly to immune repertoire derived sequences, CMC liability motifs have been eliminated from these antibodies at the library level, therefore avoiding the isolation of lead molecules carrying problematic posttranslational motifs.