Fluorescent Labeling with Cy5.5 NHS Ester: A Versatile Tool in Biological Research | GlpBio

Cy5.5 NHS ester is a fluorescent dye molecule commonly used in biological research for labeling biomolecules such as proteins, peptides, and nucleic acids. Here is a comprehensive explanation of Cy5.5 NHS ester:

Cy5.5 is a cyanine dye that emits red fluorescence when excited with an appropriate light source. It belongs to the family of cyanine dyes, which are known for their high molar absorption coefficients and quantum yields, making them ideal for fluorescence-based applications. The "5.5" in Cy5.5 refers to the specific chemical structure of the dye molecule within the cyanine dye family.

NHS ester (N-hydroxysuccinimide ester) is a chemical moiety commonly used for labeling primary amines (-NH2) in biomolecules. The NHS ester reacts with primary amines to form stable amide bonds, allowing for the covalent attachment of the dye molecule to the biomolecule of interest. This labeling method is widely used in bioconjugation reactions for fluorescent labeling.

The term "non-sulfonated" indicates that the Cy5.5 NHS ester molecule does not contain sulfonate groups. Sulfonate groups can sometimes interfere with the labeling process or affect the properties of the labeled biomolecule. By being non-sulfonated, the Cy5.5 NHS ester may offer advantages in specific labeling applications where sulfonate groups are not desirable.

Cy5.5 NHS ester is commonly used in various biological and biochemical applications, including fluorescence microscopy, flow cytometry, protein labeling, cell imaging, and molecular biology studies. The red fluorescence emitted by Cy5.5 allows for sensitive detection and imaging of labeled biomolecules in complex biological samples.

When using Cy5.5 NHS ester for labeling experiments, it is important to consider factors such as dye-to-protein ratio, reaction conditions (pH, temperature), and purification methods to ensure efficient and specific labeling of the biomolecule. Optimization of labeling conditions is crucial for obtaining reliable and reproducible results.

In summary, Cy5.5 NHS ester is a versatile fluorescent dye reagent that enables specific and sensitive labeling of biomolecules for various biological research applications. Its unique properties make it a valuable tool for visualizing and studying biological processes at the molecular level.
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