Experiment 345 - Exploring the Genetics of Taste: PCR Analysis of the PTC Gene PCR: Student Protocol

PTC is a compound with a bitter taste. The ability to taste PTC is determined by a single gene and can be confirmed by testing PTC paper. The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of students' PTC gene that links detection of the characteristic taste of PTC paper.

This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained. This video shows all of the steps in the student protocol.

For more information, or to download the experiment, go to www.edvotek.com/345

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