Cytek Aurora Training Part 2: Reference Controls

Aurora User Training Spectral Flow Cytometry

PART 2 - Reference Controls
0:00 Intro
0:44 Overview of rules for reference controls
1:25 Rule 1 sufficient number of events
5:48 Rule 2 clear separation of positive
7:03 Rule 3 matching autofluorescence
10:09 Rule 4 fluorophore brightness
12:22 Rule 5 fluorophore signature
17:12 Quiz
20:29 Tips for manufacturing controls
22:22 Should you use beads or cells?

For more information on when you can reuse old controls, see this video:    • How to combine multiple aurora experi...  

Advanced tips for setting up the universal negative feature in SpectroFlo:    • Advanced Tips for Organizing Unstaine...  

Tutorial on determining if beads or cells are best to unmix:    • Advanced Unmixing in SpectroFlo (Cyte...  

This is a comprehensive course for researchers who would like to use the Cytek Aurora. This course assumes that researchers are already familiar with conventional flow cytometry. Slides can be downloaded here: https://drive.google.com/file/d/1wF9-... More information and resources can be found here: https://voices.uchicago.edu/ucflow/sp...

View the full training course:    • Cytek Aurora Training Course  

Cytometry and Antibody Technology Facility
University of Chicago

I have no affiliation with Cytek Biosciences.