Скачать или смотреть Electrophoresis and Hybridization Technology | ASCP MLS Prep

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From DNA gels to FISH and microarrays—unlock the core techniques of electrophoresis and hybridization in molecular biology.

Explore the essential electrophoresis and hybridization technologies that form the backbone of molecular biology and clinical diagnostics. This lesson covers how DNA, RNA, and proteins are separated, analyzed, and detected, as well as how advanced hybridization tools reveal genetic information with precision.

What you’ll learn:

Electrophoresis of nucleic acids

Agarose/polyacrylamide gels: separation by size and charge

Capillary electrophoresis: automated, high sensitivity, high resolution

Pulsed-field gel electrophoresis (PFGE): analysis of very large DNA fragments, outbreak genotyping

Hybridization blot techniques

Southern blot: DNA detection

Northern blot: RNA detection

Western blot: protein detection with antibodies

Southwestern blot: DNA-binding proteins

Dot/slot blots: rapid nucleic acid screening

Reverse dot blots: allele-specific probes for genotyping

Advanced hybridization applications

Stringency: salt, heat, and formamide effects

DNA microarrays (DNA chips): genome-wide expression profiling, diagnostics, and pharmacogenetics

Modern tools

FISH: fluorescent in-situ hybridization for DNA/chromosome mapping

CGH: comparative genomic hybridization for copy number variation

DNA sequencing: Maxam-Gilbert, Sanger, pyrosequencing, and next-gen sequencing

Gene cloning: recombinant DNA technology for protein expression

DNA polymorphisms: RFLPs, VNTRs, STRs, SNPs, Y-STRs, and mitochondrial DNA markers

Why it matters: These methods are vital for diagnostics, genetic research, and biotechnology—linking lab science to clinical and forensic applications.

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