MRM Mass Spectrometry of Peptides Applied to Meat Authentication| Protocol Preview

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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication - a 2 minute Preview of the Experimental Protocol

Yvonne Gunning, Andrew D. Watson, Neil M. Rigby, Mark Philo, Joshua K. Peazer, E. Kate Kemsley
Institute of Food Research, Analytical Sciences Unit; Institute of Food Research,; University of East Anglia, School of Chemistry;

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

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