Скачать или смотреть Cryptococcus capsule in Giemsa stained smear

Giemsa stained slide of Cryptococcus neoformans showing clear capsule
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Principle of Giemsa stain
Giemsa contains Methylene blue(AzureII)/Eosin. Methylene blue on oxidation produces colored compounds termed ‘Azure’ that have ability to combine with Eosin. Methylene blue azure is blue violet and stain acidic cell components while eosin is red and stains basic cell components.

Procedure of Giemsa stain
For bone marrow imprints and smears
Smears are fixed in methanol for 30 minutes.

Smears are stained in working Giemsa solution for 20 minutes

Wash under running tap water for 5 minutes.

Air dry smears, clear in xylene and mount in D.P.X.