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High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots - a 2 minute Preview of the Experimental Protocol

Thomas B. Jacobs, Gregory B. Martin
Boyce Thompson Institute for Plant Research,; Cornell University, Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science;

Using DNA assembly, multiple CRISPR vectors can be constructed in parallel in a single cloning reaction, making the construction of large numbers of CRISPR vectors a simple task. Tomato hairy roots are an excellent model system to validate CRISPR vectors and generate mutant materials.

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