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Complementation Test

A complementation test is a genetic test used to determine whether two mutations are in the same gene or different genes.

Purpose:

1. *Gene Identification*: To identify whether two mutations are allelic (in the same gene) or non-allelic (in different genes).
2. *Gene Function*: To study gene function and interactions.

How it Works:

1. *Crossing Mutant Strains*: Two mutant strains with different mutations are crossed.
2. *Analyzing Progeny*: The progeny are analyzed for the presence or absence of the mutant phenotype.

Interpretation:

1. *Complementation*: If the progeny exhibit a wild-type phenotype, the mutations are in different genes (non-allelic).
2. *No Complementation*: If the progeny exhibit a mutant phenotype, the mutations are in the same gene (allelic).

Applications:

1. *Genetic Mapping*: Complementation tests can be used to map genes and determine their relationships.
2. *Gene Cloning*: Complementation tests can be used to identify and clone genes.

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