ACT-PRESTO: Biological Tissue Clearing & Immunolabeling Methods-Volume Imaging l Protocol Preview

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ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging - a 2 minute Preview of the Experimental Protocol

Eunsoo Lee, Woong Sun
Korea University College of Medicine, Department of Anatomy;

ACT-PRESTO (active clarity technique–pressure related efficient and stable transfer of macromolecules into organs) enables rapid, efficient, but inexpensive tissue clearing and rapid antibody penetration into cleared tissues by passive diffusion or pressure-assisted delivery. Using this method, a wide range of tissues can be cleared, immunolabeled by multiple antibodies, and volume-imaged.

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