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Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant - a 2 minute Preview of the Experimental Protocol

Gauri Tendulkar, Phillip Grau, Patrick Ziegler, Alfred Buck, Sr., Alfred Buck, Jr., Andreas Badke, Hans-Peter Kaps, Sabrina Ehnert, Andreas K. Nussler
Eberhard Karls Universität Tübingen, Siegfried Weller Institute for Trauma Research at the BG Trauma Center; BG Trauma-Center, Department of Orthopaedics; Buck GmbH and Co.KG,;

Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.

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