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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS - a 2 minute Preview of the Experimental Protocol

Claudia A. Simões-Pires, Vincent Zwick, Sylvian Cretton, Muriel Cuendet
Universités des Geneva–Lausanne (EPGL), Section des Sciences Pharmaceutiques;

The present method serves to identify isoform-specific inhibitors of histone deacetylases (HDAC) in HeLa cells by the UHPLC-MS analysis of multiple substrates. This is an antibody-free method developed to reflect HDAC1 and HDAC6 activity in the living cell environment, in contrast to single-isoform cell-free assays.

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