Скачать или смотреть CELL CULTURE: -Some of the cultures became contaminated with visible, fuzzy mold colonies. List bri…

CELL CULTURE: -Some of the cultures became contaminated with visible, fuzzy mold colonies. List briefly three precautions/procedures one should employ to reduce the possibility of such contamination. -A scientist froze 2x10^6/mL CHO cells in an appropriate freezing media. The next week, upon rapid thawing and proper resuspension in complete media, only 1/4 of the cells were found to be alive using the trypan blue dye exclusion method in counting. What could be a possible reason for so many dead cells? -Give one hint for success when using the trypan blue dye exclusion method.

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