Скачать или смотреть 10× TBST Tris Buffered Saline with Tween-20 G0004

TBST buffer, or TBS with Tween-20, is an isotonic buffer salt solution commonly used in biology. It consists of Tris-HCl to form a stable pH buffer system. NaCl provides isotonic conditions, and Tween-20 as a detergent can increase the elution capacity of buffer. It can be used in Western Blot experiments to wash off non-specific binding antibodies and other reagents, as well as in immunofluorescence and immunohistochemistry experiments, preparation of blocking solution, preparation of primary antibody or secondary antibody, washing after incubation of primary antibody or secondary antibody, etc., to reduce background and enhance signal-to-noise ratio.

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