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Isolation of Stem Cells from Human Pancreatic Cancer Xenografts - a 2 minute Preview of the Experimental Protocol

Zeshaan Rasheed, Qiuju Wang, William Matsui
Johns Hopkins University School of Medicine, Sidney Kimmel Comprehensive Cancer Center;

Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.

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