Скачать или смотреть Immunogold Labeling of Synaptic Proteins in Hippocampal Slice cultures | Protocol Preview

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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures - a 2 minute Preview of the Experimental Protocol

Ling Zhong, Joshua C. Brown, Clive Wells, Nashaat Z. Gerges
Medical College of Wisconsin , Department of Cell Biology, Neurobiology and Anatomy; Medical College of Wisconsin , Department of Microbiology and Molecular Genetics;

The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve structures and antigens in the CNS to study and characterize synaptic proteins in rat hippocampal CA1 pyramidal neurons.

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