Скачать или смотреть DNA Automation with Nano-Structured Ceramics

2022.10.20 Mario Blanco, NANOPEC Inc.

This presentation is made available by the The Nanotechnology Applications and Career Knowledge (NACK).
Information on the NACK Network can be found at: https://www.cneu.psu.edu/ and https://nano4me.org

Fully synthetic oligonucleotides are critical in nearly all disciplines of modern biology and precision medicine. Synthetic Biology, cell-free DNA (cfDNA) diagnostics, mRNA vaccines, interference RNA cancer therapeutics, self-amplifying saRNA. Access to abundant, high quality synthetic DNA and RNA at gram scales overnight could be a game changer for the future of healthcare and personalized medicine 1) reducing the time for the discovery of safe and effective biologics, 2) significantly increasing the clinical Phase I pipeline success and 3) reaching profitability years in advanced to recover R&D costs.

Typical commercial offerings for 500 ng of a single lyophilized gene take from 5 to 15 business days and cost over $2500 to produce. For even partial combinatorial libraries of mRNA biologics this process can become time consuming and prohibitable expensive. We will discuss how the use of nano-structured ceramic films enables the synthesis of hundreds of DNA genes in gram quantities overnight. Furthermore, we will describe how these nano-structured ceramics can be manufactured without the use of expensive, currently highly backlogged, nanofabs through the use of conventional physical and chemical means.

Table of Contents:
00:00 DNA Automation with Nano-Structured Ceramics
00:27 This webinar is hosted by
00:49 Presenter
01:06 DNA Automation with Nano-Structured Ceramics
02:31 DNA Synthesis Automation Goals
08:36 The fundamentals The applications Comparative advantage How it works Next level automation
09:14 What is a nanometer ?
11:47 Small means fast: electronic chips automation analogy
13:45 Scale of DNA Automation: Present and Future
16:58 Untitled: Slide 10
17:48 Simplified Workflow
20:24 The core of the technology is a patented nanoporous ceramic enabling precise DNA and RNA synthesis through pore size control
23:00 DNAReax™ can transform the synthetic biology market
25:51 Nanostructured Ceramic vs CPG
28:10 Questions?
34:57 One of the world's largest gene banks is already using DNAReax™
37:10 Competitive analysis: automation outperforms current technologies
40:43 Current focus is on early adaptors and applications in biological therapeutics
41:40 Platform is unique in being able to create pre-clinical amounts of cell free, animal free DNA overnight
43:04 Collaboration with pharma client developing DNA based therapeutics
44:05 Proprietary DNA Spacing on Nano-porous Substrate
44:32 nanopec protects IP: combination of patents and trade secrets
44:57 Thank you!

This resource and related downloads can be found at: https://nanohub.org/resources/36666