Fucosylated HMOs Analysis by Genetically Encoded Biosensors | Protocol Preview

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Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors - a 2 minute Preview of the Experimental Protocol

Fatima Enam, Thomas J. Mansell
Iowa State University, Department of Chemical and Biological Engineering;

We describe here the high-throughput detection and quantification of fucosylated human milk oligosaccharides (HMOs) using a whole-cell biosensor. We also demonstrate here, the adaptation of this platform towards analysis of HMO production strains, focusing on improving the signal to noise ratio.

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