Скачать или смотреть Developing an Assay for Break Induced Replication in Arabidopsis - UC Davis Research Rockstars 2021

A look into my research in the Comai lab at UC Davis with the help of pasta!

Project description:
Break induced replication (BIR) is a mechanism for repair of double stranded DNA breaks. It involves invasion of a homologous chromosome by the broken DNA, which uses it as a template for replication. The repaired part of the chromosome will contain genes from the homologous chromosome, as well as numerous mutations. In yeast it has been observed that BIR results in mutations with patterns similar to those seen in human cancers. BIR has not been studied in-depth, so there is still much to be discovered about the exact mechanisms and reasons for mutations. The Comai lab recently characterized what is believed to be BIR in potato, the first documented case in plants. In my research I am working on an assay to make BIR occur at high rates in Arabidopsis plants using CRISPR. We are integrating the gene for Cas-9 protein and a gRNA which will cut DNA at a specific site on a chromosome. A gene for branched leaf hairs will be tracked, and loss of this gene will result in unbranched leaf hairs and indicate that BIR has occurred in that area. Having a reliable way to induce BIR can pave the way for further research.

Special thanks to Kirk Amundson, Luca Comai, Traci DeMarco, Joe DeMarco, Sarah Suriano, and all of my wonderful friends and family for their support.

*No pasta was harmed in the making of this video*