Скачать или смотреть DNA glycosylase attacks N-Glycosidic bond in DNA during Base excision repair

Structure of Nucleotide is very important to understand the DNA, RNA, ATP, NAD, NADP, FMN, FAD, cAMP, cGMP etc. Understanding of bonds in the structure of Nucleotide is very important because enzyme deficiencies related especially to action on N-Glycosidic bond associated with human disorders. Here we are discussing two enzymes: N-glycosidase serve function in Base exision repair mechanism of DNA and Purine Nucleoside phosphorylase (PNP) -deficiency of which is the cause of 4% of SCID (Severe combined immunodeficiency)

6:15 I have told that PNP converts nucleoside into base and ribose 5 phosphate. In fact ribose-1-phosphate is the product. The two main pentose phosphates, ribose-5-phosphate and ribose-1-phosphate, are readily interconverted by the action of phosphopentomutase. Ribose-5-phosphate is the direct precursor of 5-phosphoribosyl-1-pyrophosphate, for both de novo and 'salvage' synthesis of nucleotides.