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Bacterial Detection & Identification Using Electrochemical Sensors - a 2 minute Preview of the Experimental Protocol

Colin Halford, Vincent Gau, Bernard M. Churchill, David A. Haake
Veterans Affairs Greater Los Angeles Healthcare System, Research Service; University of California, Los Angeles , Department of Urology, The David Geffen School of Medicine; GeneFluidics,; Veterans Affairs Greater Los Angeles Healthcare System, Division of Infectious Diseases; University of California, Los Angeles , Department of Microbiology, Immunology & Molecular Genetics;

We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.

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