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Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering - a 2 minute Preview of the Experimental Protocol

Pearlie S. J. Low, Monica S. Tjin, Eileen Fong
Nanyang Technological University, School of Materials Science and Engineering;

Recombinant technologies have enabled material designers to create novel artificial proteins with customized functionalities for tissue engineering applications. For example, artificial extracellular matrix proteins can be designed to incorporate structural and biological domains derived from native ECMs. Here, we describe the construction and purification of aECM proteins containing elastin-like repeats.

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