Estimation of Serum Total Cholesterol by Enzymatic (CHOD-POD) Method || Biochemistry Practical

Estimation of Serum Total Cholesterol by Enzymatic (CHOD-POD) Method - This is the video on estimation of serum total cholesterol by cholesterol oxidase and peroxidase method with discussion of viva questions and
demonstration.
Aim and Introduction - 00:30 minutes
Importance of estimation of cholesterol - 01:05 minutes
Important biological compounds derived from cholesterol - 01:48 minutes
Principle of Cholesterol Estimation by Enzymatic Method - 02:22 minutes
Operational Procedure - 03:11 minutes
Calculation of Total Cholesterol - 03:55 minutes
Normal Range of Total Cholesterol - 04:38 minutes
Lipid Profile - 04:43 minutes
Friedewald equation - 05:15 minutes
Hypercholesterolemia - 07:25 minutes
Hypocholesterolemia - 07:51 minutes
Viva Questions - 08:11 minutes
Demonstration of Estimation of Serum Total Cholesterol by Enzymatic (CHOD-POD) Method - 11:17 minutes

Cholesterol is the most abundant steroid in the human tissue and body fluids.
It is synthesized in the liver and transported in the blood mainly in the form of LDL and HDL.
In blood, cholesterol is present in two forms:
Free (20 - 25%) and
Esterified (75 – 80%).

Important compounds derived from cholesterol:
Bile acid
1ry bile acid- Cholic acid & Chenodeoxy cholic acid
2ndry acid- Lithocholic acid & Deoxycholic acid
2. Vit.D
3. Steroid hormones


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