Tutorial MCR-ALS Imaging with CytoSpec

MCR-ALS (Multivariate Curve Resolution - Alternating Least Squares) is one of the most useful data analysis and machine learning methods. It can be used to recover pure response profiles (spectra, concentration, time, elution profiles, etc.) of chemical constituents of unresolved mixtures.

In hyperspectral imaging (HSI) application MCR-ALS assumes that all pixel spectra constituting a HSI can be considered linear combinations of pure component spectra. In this way each of the components is weighted by its concentration at any given [x,y] position of the pixel spectrum. MCR-ALS imaging is an iterative procedure that aims at finding the pure component spectra as well as the corresponding (spatial) concentration profiles.

This video provides a general introduction in MCR-ALS imaging along with the necessary spectral preprocessing procedures. For this purpose, in this video tutorial Raman hyperspectral imaging data are processed exemplarily.

If you are interested in learning more about MCR-ALS imaging, see
https://www.cytospec.com/multivar.php...

General information about the software used can be found at the CytoSpec website: https://www.cytospec.com/ftir.php#top