Modified Two-Photon Microscope: Application To Tracking Of Intracellular Vesicles l Protocol Preview

Watch the Full Video at https://www.jove.com/v/51794/3d-orbit....

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles - a 2 minute Preview of the Experimental Protocol

Andrea Anzalone, Paolo Annibale, Enrico Gratton
University of California, Irvine, Biomedical Engineering, Laboratory for Fluorescence Dynamics;

In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.

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