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Dengue NS1 IgG IgM Combo Rapid Test Procedure.

This video describes the #procedure and performance of #Dengue Combo Card Test NS1 Antigen and IgG + IgM Antibodies from Anamol Laboratories Pvt. Ltd. India.

👉🔴Intended Use🔴
Test for detection of IgG and IgM antibody against Dengue virus in human serum and plasma samples.

👉🔴Introduction:
Dengue virus, a virus belonging to the Flavivirus group of viruses, is one of the most significant mosquito-born diseases in the world in terms of morbidity and mortality. There are four known serotypes of dengue. Symptoms of dengue fever include high fever, headache, muscle pain and skin rash. The complications often associated with this infection are dengue hemorrhagic fever or dengue shock syndrome. The immune response to this virus includes the production of IgM antibodies by the 5th day of symptoms, which remain in the circulatory system for 30-60 days. IgG antibodies appear by the 14th day of infection and persist for life. A secondary infection often results in high fever and, in many cases, initiates hemorrhagic events and circulatory failure. A secondary infection also induces an IgM antibody response after 20 days of infection and IgG antibodies rise within 1-2 days after the onset of symptoms.

👉🔴Method Principle:
First a specimen is dispensed with sample buffer, the Gold antigen conjugate will bind to anti-Dengue IgG and IgM antibodies in the specimen sample which in turn will bind with Anti-Human IgG and Anti-Human IgM coated on the membrane as two separate lines in the test region as the reagent move across the membrane. The anti-Human antibodies on the membrane will bind the IgG or IgM antigen complex at the relevant IgG and or IgM test lines causing pale or dark pink lines to form at the IgG or IgM region of the test membrane. The intensity of the lines will vary depending upon the amount of antibody present in the sample. The appearance of pink line in a specific test region (IgG or IgM) should be considered as positive for that particular antibody type (IgG or IgM).


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