Column chromatography - gel filtration chromatography lecture

this column chromatography lecture video explains about the protein gel filtration chromatography technique principle. This is also known as molecular exclusion chromatography or gel filtration chromatography. In this chromatography process molecules are separated based on their size. Mixture of molecules are passed through a polymer network made with agarose gel. Large molecules will not enter into the pores created by the agarose polymer so they will elute faster and the smaller molecules will be trapped inside the agarose polymer as a result they will take more time to come out of the column.
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